Medium sterilization method - Database & Sql Blog Articles

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What is medium sterilization? What are the methods of sterilizing the medium? Today Shanghai Hengyuan shares the method of sterilizing the medium with you.
First, the main principle of heat sterilization
Microbial thermal resistance
2. Logarithmic residual law
3. Calculation of theoretical sterilization time
4. Selection of sterilization temperature 2. Sterilization method
1. High pressure steam sterilization: High pressure steam sterilization is suitable for sterilization of high temperature resistant medium, inoculating equipment and distilled water. The medium is mixed with various bacteria in the process of preparation, and should be sterilized immediately after being dispensed. At least the sterilization work should be completed within 24 hours. When sterilizing, it is generally under the pressure of 0.105 MPa, and when the temperature is 121 ° C, it can be sterilized for 15 to 30 minutes. The disinfection time should not be too long, nor can it exceed the specified pressure range. Otherwise, organic substances, especially vitamins, will decompose at high temperatures, losing nutrients, and also deteriorating, discoloring, and even hardening the medium. Distilled water or tap water is placed in a triangular flask. The water content is generally not more than 2/3 of the bottle. The seal is wrapped with kraft paper or sulfuric acid paper, and then sterilized in a pressure cooker to be sterile water. The sterilized medium can be pre-cultured for 3 days in the culture chamber. If there is no pollution, the sterilization is proved to be thorough and can be used. The medium that is not used temporarily is preferably stored at 10 °C. The medium containing IAA or GA3 should be used up within 1 week, and the other medium should not be more than 1 month at most.
2, filter sterilization: some plant growth regulators and organic matter, such as IAA, GA3, ZT, CM, etc., easily decompose when exposed to heat, can not be sterilized with the medium, but use a bacteria filter to filter out the impurities bacteria. Bacterial filters and filters (0.45 μm pore size) are autoclaved prior to use. The filtered solution should be added to the medium immediately. If it is a liquid medium, it can be added when the medium is cooled to 30 ° C. If it is a solid medium, it must be added before the medium is solidified (50-60 ° C), and shaken. The solution is mixed well with other ingredients.
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